Project information
The structure of interphase nucleus and its changes after irradiation
- Project Identification
- GA202/97/0874
- Project Period
- 1/1997 - 12/1999
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Informatics
- Cooperating Organization
-
Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
- Responsible person Ing. Václav Kroha, CSc.
In spite of the fact that the methods for the investigation of gene location are available for several years, the structure of interphase nucleus is poorly known and radiation induced changes in the space distribution of interphase chromosomes have been investigated only in several our experiments. Therefore, the main objective of the proposed research in the determination of 2D and 3D distributions of some chromosomes and cancer-related genes in interphase nuclei of several humans cell lines and the in
Publications
Total number of publications: 10
2000
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Influence of cell fixation on chromatin topography
Analytical Biochemistry, year: 2000, volume: 282, edition: 1
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Nuclear topography of the c-myc gene in human leukemic cells
Gene, year: 2000, volume: 244, edition: 1
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The influence of the cell cycle, differentiation and irradiation on the nuclear location of the ABL, BCR and C-MYC genes in human leukemic cells
Leukemia Research, year: 2000, volume: 24, edition: 3
1999
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Advances in computer analysis of radiation-induced changes in human genome
Fundamentals for the assesment of risks from environmental radiation, year: 1999
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Differences in the induction of apoptosis after treatment with gamma-irradiation and C2-ceramide
Fundamentals for the assesment of risks from environmental radiation, year: 1999
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High-resolution cytometry of FISH dots in interphase cell nuclei
Cytometry, year: 1999, volume: 36, edition: 4
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Chromatin structure and its cell cycle kinetics in intact and irradiated cell nuclei
Fundamentals for the assesment of risks from environmental radiation, year: 1999
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The topological organization of chromosomes 9 and 22 in cell nuclei has a determinative role in the induction of t(9,22) translocations and in the pathogenesis of t(9,22) leukemias
Chromosoma, year: 1999, volume: 108, edition: 7
1998
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Semi-automated FISH-stained interphase nuclei analysis in human genome studies
Fluorescence Microscopy and Fluorescent Probes, year: 1998
1997
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Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia
Human Genetics, year: 1997, volume: 100, edition: 5/6