Project information
Early diagnostics of leukemia using DNA-microchip technology and by monitoring of epigenetic changes
- Project Identification
- GP301/04/P136
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA-microchipes, leukemia, epigenetic changes, chromatin structure
Using DNA-microchip technology expression maps in cells of bone marrow or immobilized blood of patients with different types of leukaemia at different stages will be obtained and compared with results obtained from cells without effect of cancer. The differences found by comparing expression maps of cells with various level of progression and differentiation will help us to the identify primary, yet known, but also new genes whose false function contributes to the disease development.
Also it should be clarified how the changes of activity of these genes reflect the activity of other known or unknown genes and what is the location of these genes on corresponding chromosomes. For epigenetic changes FISH and high-resolution cytometry will be used. Chromosomes with modulated level of expression will be interrogated structural-epigenetic-study. Changes in structure of chromatin in the cells of patients with leukaemia in the various stages of diseases in contrast to non-cancer cells will be evaluated
Results
Publications
Total number of publications: 13
2005
-
Sledování změn genové exprese u pacientů s kolorektálním karcinomem pomocí cDNA microarrays technologie a jejich diagnostika pomocí transkripčních map
Year: 2005, type: Conference abstract
-
Transcriptional profiling of human CD34+ cells during in vitro differentiation
Year: 2005, type: Conference abstract
2004
-
Clustering of genes with similar regulation patterns during monocyte and granulocyte differentiation of HL-60 cells
Biophysics of the Genome, year: 2004