Project information
The efect of methylation and acetylation on chromatin structure
- Project Identification
- GP202/03/D033
- Project Period
- 9/2003 - 8/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
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Faculty of Informatics
- Ing. Magdalena Skalníková, CSc.
- Keywords
- DNA Methylation, Acetylation Histones, Chromatin Structure, FISH
Biochemical modifications of DNA and histones (especially methylation and acetylation) belong to mechanisms of epigenetic regulation which influence chromatin structure with regards to its transcriptional activity/inactivity. Modifications of histones an DNA exist on parallel pathway, however the mutual relationship between individual epigenetic heredity mechanisms are not yet clear. In our experiments the acetylation status of histones on metaphase chromosomes of humid blood cells and methylation status of DNA and histones and will be studied. The effect of hypomethylation and hyperacetylation drugs on the level of methylation/acetylation status of histones and DNA will also be studied.
The effect of these biochemical modifications will be evaluated with regards to the higher-order chromatin structure of blood cells. The
selected metaphase chromosomes according to the content of CpG islands will be visualised using fluorescence in situ hybridization and indirect immunofluorescence method.
Results
Publications
Total number of publications: 5
2007
-
Distinct patterns of histone methylation and acetylation in human interphase nuclei
Physiological Research, year: 2007, volume: 56, edition: 6
2006
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Automatic Detection of Histones on Chromosome X in Images of Cell Nuclei
Clinician and Technology (Lékař a technika), year: 2006, volume: roč. 36, edition: 2
2005
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Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases
Journal of Cell Science, year: 2005, volume: 118, edition: 21
2004
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Cytological study of DNA methylation and histone methylation patterns in human cell lines
Biophysics of the Genome, year: 2004
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Relationship of HP1 proteins to centromeric heterochromatin characterized by the presence of H3(K9) and absence of H3(K4) dimethylation
Biophysics of the Genome, year: 2004