Project information
Bacterial oxidation of inorganic sulfur substances, environmental aspects
- Project Identification
- GA525/04/1309
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- Sulfur-oxidizing bacteria; sulfide wastes; environment acidification
This project proposal investigates various aspects of biooxidation of inorganic sulfur substances using acidophilic bacteria in relation to biochemical and chemical processes taking place in sulfide wastes and other deposits. Environmental acidification and metal migration are the results of those processes. Biooxidation of sulfur substances will be studied at cell and enzyme levels. Mechanism of pyrite biooxidation will be investigated in relation to a possible formation of sulfur intermediates during the indirect oxidation process. Inhibition of elemental sulfur biooxidation by arsenic will be studied due to the arsenic occurrence in sulfide minerals (arsenopyrite) to learn its inhibitory mechanism and environmental consequences. Acidithiobacillus ferrooxidans bacteria were isolated in the past as a dominant organism in sulfide waste at Zlaté Hory participating in water acidification. The heap surface has been recultivated. The inner heap layers will be a subject of testing to learn the effect of recultivation on the occurrence of active bacteria which could take part in further environmental acidification.
Publications
Total number of publications: 24
2008
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"FiFA for RHC" - First Functional Analysis of Acidithiobacillus ferrooxidans rhodanese-like protein
XII. Setkání biochemiků a molekulárních biologů, year: 2008
2007
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Cesta od genomu k proteinu - Rhodanasy a bakterie Acidithiobacillus ferrooxidans
Študentská vedecká konferencia, year: 2007
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Kinetic constant variability in bacterial oxidation of elemental sulfur
Applied and Environmental Microbiology, year: 2007, volume: 73, edition: 11
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Purification and some properties of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans
Preparative Biochemistry & Biotechnology, year: 2007, volume: 37, edition: 4
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Rhodaneses of Acidithiobacillus ferrooxidans - gaining protein from genom
International Congress of Young Chemists YoungChem 2007, year: 2007
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Rhodanese-type enzymes of bacterium Acidithiobacillus ferrooxidans
Bulletin Československé společnosti mikrobiologické, year: 2007, volume: 2007, edition: 48
2006
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Acidophilic bacteria in sulfuric acid formation: Variability of metabolic parameters
Year: 2006, type: Conference abstract
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Biooxidation of elemental sulfur and its impacts on metabolic parameters in Acidithiobacillus ferrooxidans
X. Pracovní setkání biochemiků a molekulárních biologů, year: 2006
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Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese
Journal of Chromatography A, year: 2006, volume: 1120, edition: 1-2
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ELECTROPHORETICALLY MEDIATED MICROANALYSIS AS A TOOL FOR KINETIC STUDIES OF ENZYMES
XX. Biochemický zjazd, year: 2006