Project information
Proteome dynamics in response to increased cytokinin levels in Arabidopsis
- Project Identification
- IAA600040701
- Project Period
- 1/2007 - 12/2010
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
- Faculty of Science
- Keywords
- cytokinins, proteome, protein phosphorylation, Arabidopsis, dexamethasone-inducible gene expression system, 2-D protein electrophoresis, MALDI-TOF/TOF MS
- Cooperating Organization
-
Institute of Biophysics
- Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
- Responsible person Ing. Janette Bobálová, CSc.
Cytokinins (CKs) are known to act in combination with other plant hormones to regulate diverse developmental processes in plants. The exact molecular mechanisms of CK action in regulation of plant development are the subject of intense current research. While genome expression profiling of CK action has already been initiated by other groups elsewhere, here we propose to analyze proteome dynamics in response to increased CK levels in Arabidopsis under conditions similar to those used in monitoring genome-wide changes in gene expression. This will allow, for the first time, a comparison of the transcriptome and proteome data on highly comparable samples. Increase in CK levels will be achieved by treatment with exogenous CKs and by employing a novel dexamethasone-inducible gene expression system pOp/LhGR. A direct comparison of effects of three distinct CK classes is expected to contribute to elucidation of their individual function(s) in regulation of plant development.
Publications
Total number of publications: 18
2012
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Rapid and efficient protein enzymatic digestion: An experimental comparison
Electrophoresis, year: 2012, volume: 33, edition: 2
2011
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Accelerated enzymatic digestion for the analysis of plant proteins
Year: 2011, type: Conference abstract
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Comparative evaluation of fast protein enzymatic digestion
Year: 2011, type: Conference abstract
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Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling
Journal of Experimental Botany, year: 2011, volume: 62, edition: 3
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Rychlé a účinné enzymatické štěpení proteinů
Year: 2011, type: Conference abstract
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The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches
Year: 2011, type: Conference abstract
2010
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Effect of decreased light intensity on Arabidopsis responsiveness to increased levels of endogenous cytokinins - a proteomic analysis
Year: 2010, type: Conference abstract
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Fast and efficient protein enzymatic digestion in proteomics
Year: 2010, type: Conference abstract
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Identification of plant proteins by the combination of electromigration techniques and MS
Year: 2010, type: Conference abstract
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Novel links between temperature, calcium and cytokinin signaling identified by proteome- and phosphoproteome-wide profiling in Arabidopsis
Year: 2010, type: Conference abstract