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Project information
Proteome dynamics in response to increased cytokinin levels in Arabidopsis

Project Identification
IAA600040701
Project Period
1/2007 - 12/2010
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
  • Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
MU Faculty or unit
Faculty of Science
Keywords
cytokinins, proteome, protein phosphorylation, Arabidopsis, dexamethasone-inducible gene expression system, 2-D protein electrophoresis, MALDI-TOF/TOF MS
Cooperating Organization
Institute of Biophysics
  • Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
Institute of Analytical Chemistry of the ASCR, v. v. i.
  • Responsible person Ing. Janette Bobálová, CSc.

Cytokinins (CKs) are known to act in combination with other plant hormones to regulate diverse developmental processes in plants. The exact molecular mechanisms of CK action in regulation of plant development are the subject of intense current research. While genome expression profiling of CK action has already been initiated by other groups elsewhere, here we propose to analyze proteome dynamics in response to increased CK levels in Arabidopsis under conditions similar to those used in monitoring genome-wide changes in gene expression. This will allow, for the first time, a comparison of the transcriptome and proteome data on highly comparable samples. Increase in CK levels will be achieved by treatment with exogenous CKs and by employing a novel dexamethasone-inducible gene expression system pOp/LhGR. A direct comparison of effects of three distinct CK classes is expected to contribute to elucidation of their individual function(s) in regulation of plant development.

Publications

Total number of publications: 17

2012

2011

2010

2009

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