Publication details

Fast and efficient protein enzymatic digestion in proteomics

Authors

DYČKA Filip BOBÁĽ Pavel BOBÁLOVÁ Janette

Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description In current work we have focused on comparison of the variable performances of the fast enzymatic digestion of proteins using the different techniques: microwave technology, ultrasonic assisted protein enzymatic digestion and proteolysis accelerated by infrared radiation. To demonstrate the suitability of fast procedure, digest bovine serum albumin and in-gel tryptic digestion of proteins extracted from plants (Hordeum vulgare, Arabidopsis thaliana) were employed.
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