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Project information
Study of accumulation of selenium and its forms in the cell cycle of green algae

Project Identification
IAA600200701
Project Period
1/2007 - 12/2009
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
  • Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
MU Faculty or unit
Faculty of Science
  • Mgr. Jiří Machát, Ph.D.
  • Mgr. Jiří Čmelík, Ph.D.
  • Mgr. Eva Niedobová, Ph.D.
Keywords
selenium; selenoproteins; cell cycle; green algae
Cooperating Organization
Institute of Microbiology of the ASCR, v. v. i.
  • Responsible person RNDr. Milada Vítová, Ph.D.

We will study an uptake and accumulation of selenium by green algae (Chlamydomonas, Scenedesmus, Chlorella). Changes in ratio of low-molecular weight forms of selenium and selenoproteins at different concentrations of selenium supplied and activity of selenoproteins GHX2, TR1 in different cell cycle phases will be followed. Localization of selenium in cells will be studied at ultra-thin sections by electronmicroscopy. By testing of different growth conditions(light intensity, spectral composition of light, temperature, CO2, nutrition), the best conditions for maximal production of selenium compounds in synchronous algal cultures will be found out. The part of the project will be a selection of strains with high selenium uptake ability. Results of laboratory experiments will be tested in production photobioreactors.

Publications

Total number of publications: 9

2009

2008

2007

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