Project information
The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques
- Project Identification
- GP203/07/P471
- Project Period
- 1/2007 - 12/2009
- Investor / Pogramme / Project type
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Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- proteomic analysis, bacterial adaptation, molecular mechanisms, Paracoccus denitrificans
The goal of this study is to explore the effects of three known regulation proteins of Paracoccus denitrificans proteome during adaptation to changed environmental conditions. It is known that these proteins serve as sensors for oxygen and nitrogenous terminal electron acceptors. After construction of the sequence database for mass spectrometry protein identification based on published P. denitrificans genome sequence, a detailed complex proteomic analysis will be performed. The effects of mutation in genes for regulation proteins on the expression of other proteins in total cell lysates will be examined. The proteins will be separated by two-dimensional gel electroporesis and identified by mass spectrometry. The proteins with changed expression will be analyzed by bioinformatic tools, the functional analyses will be performed for selected proteins. A new chromatographic/mass spectrometric method will be tested for a comparison of membrane fractions protein composition. A created sequence database will be also applied for identification of proteins from previously published studies. The results obtained will be used for supplementation of public 2-D P. denitrificans proteome database (http://www.mpiib-berlin.mpg.de/2D-PAGE/).
Publications
Total number of publications: 8
2011
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Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators
Folia Microbiologica, year: 2011, volume: 56, edition: 1, DOI
2010
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Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
Biochimica et Biophysica Acta - Proteins and Proteomics, year: 2010, volume: 1804, edition: 6, DOI
2009
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Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
Journal of Proteome Research, year: 2009, volume: 8, edition: 1, DOI
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Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
Year: 2009, type: Conference abstract
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The study on Fnr-type transcription regulators functions in bacterium Paracoccus denitrificans using proteomics, transcriptomics and bioinformatics tools
Year: 2009, type: Conference abstract
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Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
Year: 2009, type: Conference abstract
2008
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Biomarker discovery in low grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
Year: 2008, type: Conference abstract
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The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level
Year: 2008, type: Conference abstract