Project information
Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors
- Project Identification
- OC 518.30
- Project Period
- 1/2000 - 1/2001
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- COST
- MU Faculty or unit
- Faculty of Science
Characterization of properties of thin bioorganic layers immobilized on the surface of metallic electrodes of piezoelectric crystals. Active and passive methods of measurements, electrochemical properties of biomolecules, amplitude and phase of response. Adsorption of thiocompounds on the surface of gold and silver will serve for stable immobilization of proteins, nucleic acids and lipid layers. Monitoring of conformation changes of biomolecules due to physical factors (temperature, pH, ionic strength). Kinetic interactions of immobilized biomolecules with complementary affinity partners as well as the effect of external conditions on conformation and stability of affinity complexes on the surface of crystals. Several methods for assay of the model compounds (pesticides, albumin, oligonucleotides) will be tested using piezoelectric biosensors; direct measurement of small molecules, competitive and sandwich affinity assays.
Publications
Total number of publications: 14
2004
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Activation of the DNA-binding ability of latent p53 protein by protein kinase C is abolished by protein kinase CK2
Biochemical Journal, year: 2004, volume: 378, edition: 11
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Generation and characterization of single-chain antibody fragments specific against transmembrane envelope glycoprotein gp46 of Maedi-visna Virus
Journal of Virological Methods, year: 2004, volume: 115, edition: 1
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Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
Journal of Virological Methods, year: 2004, volume: 117, edition: 2
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The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy
Bioelectrochemistry, year: 2004, volume: 62, edition: 1
2003
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Development of piezoelectric immunosensors for competitive and direct determination of atrazine
Sensors and Actuators B Chemical, year: 2003, volume: 91, edition: 1
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Development of the piezoelectric biosensor for acetochlor detection
Analytical Letters, year: 2003, volume: 36, edition: 10
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Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor
Supramolecular Chemistry, year: 2003, volume: 15, edition: 2
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New principle for the direct real-time monitoring of interaction of cholinesterase and its inhibitors
Biosensors & Bioelectronics, year: 2003, volume: 18, edition: 11
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Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
Journal of the Brazilian Chemical Society, year: 2003, volume: 14, edition: 4
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Recombinant single chain Fv antibodies that recognise the p25 protein of the Maedi-Visna virus
Folia Microbiologica, year: 2003, volume: 48, edition: 3