Publication details

Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases

Investor logo
Investor logo
Investor logo
Investor logo
Authors

ŠTĚPÁNKOVÁ Veronika DAMBORSKÝ Jiří CHALOUPKOVÁ Radka

Year of publication 2013
Type Article in Periodical
Magazine / Source Biotechnology Journal
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/biot.201200378
Field Biochemistry
Keywords Haloalkane Dehalogenases
Description Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. Ethylene glycol and 1,4-dioxane were shown to have the most positive impact on the enantioselectivity. The favourable influence of the co-solvents on both activity and enantioselectivity makes DbjA suitable for biocatalytic applications. This study represents the first investigation of the effects of organic co-solvents on the catalytic performance of haloalkane dehalogenases and will pave the way for their broader use in industrial processes.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info