Publication details
Detekce cystické formy Borrelia burgdorferi s.l. u pokusných jednotek BALB/c
| Title in English | Detection of the cystic forms of Borrelia burgdorferi s.l. to the experimental units BALB/c |
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| Authors | |
| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | The ability of the bacteria Borrelia burgdorferi sensu lato viable spiral form to generate cystic form has been published for many years. But the impact of the cystic forms on the immune system of the infected individuals is not explored enough so far, nor the role of this form in the process of Lyme disease is yet understood. The aim of our study was to assess the effect of the cystic forms Borrelia burgdorferi sensu lato on the immune response in treated experimental subjects Mus musculus (BALB /c). Cystic forms of different age (3, 10 and 17 days) were prepared and injected intraperitoneally into the tested mice. The effect of the cystic forms on the immune response was monitored during a five week period using the detection of specific IgG and IgM antibodies in a serum of the infected individuals by ELISA (Enzyme-linked immunosorbent assay) method. No influence of the cystic forms to the level of specific antibodies was shown during the study, by comparison of the level of antibodies generated by a control group of mice immunized by Borrelia motile viable cells and a group of mice treated by Borrelia sonnified cells, which generated antibodies. The method of cultivation of the cystic forms in BSKII (Barbour-Stoenner-Kelly) medium demonstrated the ability of these forms to reconvert back into the viable spiral forms, such ability depends on the age of the cysts. With increasing age, the ability of the cysts to return to the spiral form declines. Similarly a patented method Dualdur® was carried out, where motile forms of bacteria were detected in the blood of the experimental animals. The difficulty to detect the cystic forms in samples either by ELISA or other molecular and microbiological methods hinders the diagnosis and treatment of Lyme disease. From this perspective, it is obvious that a close attention towards etiology of the cystic forms of Borrelia burgdorferi sensu lato is needed for further research. |