Publication details
Detekce protilátek proti některým patogenním mikroorganismům způsobujícím zoonotická onemocnění
| Title in English | Detection of antibodies against some pathogenic microorganisms causing zoonotic diseases |
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| Authors | |
| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Pathogenic microorganisms Borrelia burgdorferi sl, Francisella tularensis, Coxiella burnetii, Leptospira grippotyphosa and Leptospira pomona are agents of zoonotic diseases, diseases transmitted from animals to humans through vectors (eg. ticks) or through water contaminated with feces of reservoir animals. In this work we deal with the possible occurrence of these pathogens in small rodents and insectivores. The aim of this research is to detect the prevalence of certain pathogenic microorganisms that cause infectious diseases such as Lyme disease, tularemia, leptospirosis and Q-fever in selected locations in the Czech Republic (CHKO Moravský kras and CHKO Poodří). Samples were collected by trapping rodents in two locations in the Czech Republic (CHKO Moravský kras and CHKO Poodří). As the sample was used serum and blood of trapped rodents. To detect the presence of IgG and IgM antibodies against antigens of these pathogens was used ELISA (enzyme-linked immunosorbent assay), antileptospiral antibodies were detected using an indirect method microscopic agglutination test, all results were analyzed statistically. In 2013, 95 were captured rodents and insectivores of the genus Apodemus, Sorex, Microtus, Clethrionomys and Talpa. Positive for the presence of antibodies against antigens grippotyphosa L. and L. pomona was 8.7%. Positivity (positive and borderline positive results) IgG antibodies against Borrelia burgdorferi sl was 13%, and 19.6% of the IgM class. The positivity of IgG antibodies against Francisella tularensis was 7.6% and IgM 9.8%. The positivity of IgG antibodies against Coxiella burnetii was 9.8% and IgM antibodies was 27.2%. After comparing the sensitivity of whole-cell and recombinant antigen was found with recombination ELISA antigen sensitive and accurate. At both locations, the rodents involved in the life cycle investigated pathogenic microorganisms. |
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