Cytotoxic Constituents from Lobaria scrobiculata and a Comparison of Two Bioassays for Their Evaluation

• Authors: SCHINKOVITZ Andreas; KANG Avinash Kaur; URBAN Ernst; ZEHL Martin; PÁCHNIKOVÁ Gabriela; WANG Yuehong; KRETSCHMER Nadine; SLANINOVÁ Iva; PAULI Guido F.; FRANZBLAU Scott G.; KRUPITZA Georg; BAUER Rudolf; KOPP Brigitte
• Year of publication: 2014
• Type: Article in Periodical
• Magazine / Source: Journal of Natural Products
• MU Faculty or unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1021/np4008574
• Field: Pharmacology and pharmaceutical chemistry
• Keywords: USNEA-LONGISSIMA; LICHEN; ACID; PULMONARIA
• Description: Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.