Publication details
Genotypové odlišnosti technologických kmenů kvasinek rodu Saccharomyces
| Title in English | Genotypic differences of technological yeast strains of genus Saccharomyces |
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| Authors | |
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | Čo nového v mikrobiológii - Zborník krátkych článkov |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | ale and lager yeast; genotype |
| Description | Lager brewing yeasts are hybrid polyploid microorganisms. This characteristic causes problems in their identiffication, especially of technologically different species S. pastorianus and S. cerevisiae. Wine yeasts are mostly diploid and belong to the species S. cerevisiae and S. bayanus. In present work were analysed at least 40 brewing, 8 wine and 3 type yeast strains by PCR and RFLP methods. We choosed flocculating genes, gene HIS4, region ITS, RRL2 and YBR033w as target for differentiation. The growth at different temperatures (30, 37 a 40°C) were monitored. Unfortenately, the differences among genes do not often reflect the differences among species in hybrid industrial yeast strains. The identification and nomenclature of hybrid strains is still very complex and until now nonuniform. |
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