Publication details

Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients' Swabs

Authors

KOMINKOVA Marketa MICHALEK Petr CIHALOVA Kristyna GURAN Roman CERNEI Natalia Vladimirovna NEJDL Lukáš SMERKOVA Kristyna DOSTALOVA Simona CHUDOBOVA Dagmar HEGER Zbynek VESELÝ Radek GUMULEC Jaromír KYNICKY Jindrich XHAXHIU Kledi ZITKA Ondrej ADAM Vojtěch KIZEK Rene

Year of publication 2015
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.mdpi.com/1422-0067/16/4/7210
Doi http://dx.doi.org/10.3390/ijms16047210
Field Biochemistry
Keywords buthionine sulfoximine; Escherichia coli; glutathione; infections; swabs
Description In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (gamma-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.

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