Publication details
Combined confocal Raman spectroscopy and optical coherence tomography for tissue analysis
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| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
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| Description | Various combinations of different noninvasive diagnostic optical methods for tissue imaging and analysis have come into high demand in the last few years. The combination of Raman spectroscopy and optical coherence tomography allows to determine the molecular structure of a sample and thus differentiate between tissues and abnormalities, and at the same time image its depth profile by cross-sectional scanning on the micron scale. We report an analysis of three different porcine tissues - skin, cartilage and tendon - using an in-house developed apparatus combining confocal Raman spectroscopy and optical coherence tomography. The differences between individual samples were determined on the basis of Raman spectra as well as on the basis of OCT cross section images. Raman spectroscopy was used to monitor water loss during these in vitro measurements. Based on the obtained OCT images, Raman measurements were conducted for each sample at various depths to find out possible changes in their molecular structure. |