Publication details

Comparison of Gold CD-R Types as Electrochemical Device and as Platform. for Biosensors

Authors

FOGUEL Marcos Vinicius DOS SANTOS Glauco FERREIRA Antonio MAGNANI Marina MASCINI Marcello SKLÁDAL Petr BENEDETTI Assis YAMANAKA Hideko

Year of publication 2016
Type Article in Periodical
Magazine / Source Journal of the Brazilian Chemical Society
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.5935/0103-5053.20150308
Field Biophysics
Keywords Amperometric sensor; Electrochemistry; Atomic force microscopy
Description The construction of gold electrodes using 3 kinds of gold recordable compact discs (CDtrodes) and characterization by electrochemical and spectroscopic techniques are herein reported. Following a surface pretreatment in 0.5 mol L-1 sulfuric acid (H2SO4), the electrochemical experiments were performed in the presence of redox couple ferro/ferricyanide [Fe(CN)(6)](3-/4-). The immobilization of an oligopeptide bio-inspired by acetylcholinesterase onto the CDtrode was also evaluated. Surface characterization was done by cyclic voltammetry, electrochemical impedance spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy and atomic force microscopy. CDtrodes are known to be of low cost, fast and simple construction. They are suitable for immobilizing biological molecules and could be used as disposable transducer for the construction of biosensors.

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