Visualization of stable ferritin complexes with palladium, rhodium and iridium nanoparticles detected by their catalytic activity in native polyacrylamide gels

• Authors: PEKAŘÍK Vladimír; PEŠKOVÁ Marie; GURAN R.; NOVÁČEK Jiří; HEGER Z.; TRIPSIANES Konstantinos; KUMAR Jitender; ADAM V.
• Year of publication: 2017
• Type: Article in Periodical
• Magazine / Source: Dalton Transactions
• MU Faculty or unit: Faculty of Medicine
• Web: http://dx.doi.org/10.1039/C7DT02818K
• Doi: http://dx.doi.org/10.1039/C7DT02818K
• Field: Biochemistry
• Keywords: stable ferritin complexes

Attached files

• Pekarik_et_al_Dalton_Transactions_2017_print_version.pdf

• Description: The reductive discoloration of azo dye, Congo red, catalyzed by noble metal nanoparticles was used to visualize protein–metal complexes in native polyacrylamide gels after counterstaining with Coomassie blue. This technique was used to characterize the synthesis of palladium, rhodium and iridium nanoparticles encapsulated in Pyrococcus furiosus ferritin.

Related projects

• Czech Infrastructure for Integrative Structural Biology
• CEITEC 2020