Publication details
Dissimilatory sulfate reduction of intestinal sulfate-reducing bacteria and their association with colitis development
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| Year of publication | 2018 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Intestinal sulfate-reducing bacteria (iSRB) are strict anaerobic microorganisms, which are generally isolated from environmental sources such as soils, lakes, marshlands; they also make part of animal and human large intestine microbiome. They obtain energy by conducting dissimilatory sulfate reduction, resulting in the release of a high quantity of hydrogen sulfide. There are lot of enzymes involved in this process, including ATP sulfurylase, APS reductase and sulfite reductase. SRB may have had a role in human inflammatory bowel diseases (IBDs). The number of cases of IBD is growing in the recent years and the etymology is still unclear. IBD is mainly used to describe two main conditions such as ulcerative colitis (UC) and Chron’s disease. They are also found with their products of metabolism in feces from people with symptoms of gut inflammation (bloody diarrhea, weight loss, and increased intestinal permeability). Although, SRB as part of the microbiota of the large intestine, their increased level may contributes to development of the colitis, associated mainly with hydrogen sulfide production. The impairment of the functions of the intestinal epithelium due to a high concentration of hydrogen sulfide would lead to cell death and chronic inflammation. The dissimilatory sulfate reduction and enzymatic activity of iSRB associated with IBD have not been investigated. Intestinal SRB samples isolated from rodents (mice and rats) with UC had higher sulfate reduction, sulfide production, as well as specific enzymatic activity for ATP sulfurylase compared to healthy controls. Also, the enzymatic activity of APS reductase was measured low along with low differences between healthy controls and samples with UC. Nonetheless, sulfite reductase, which produce hydrogen sulfide from sulfite is specific for every examined sample. |