Publication details

Multicenter prospective study on multivariant diagnostics of autoimmune bullous dermatoses using the BIOCHIP technology

Authors	VAN BEEK Nina KRÜGE Stine FUHRMANN Tarek LEMCKE Susanne GOLETZ Stephanie PROBST Christian KOMOROWSKI Lars DI ZENZO Giovanni DMOCHOWSKI Marian DRENOVSKA Kossara HORN Michael JEDLIČKOVÁ Hana KOWALEWSKI Cezary MEDENICA Ljiljana MURRELL Dedee PATSATSI Aikaterini GELLER Shamir UZUN Soner VASSILEVA Snejina ZHU Xuejun FECHNER Kai ZILLIKENS Detlef STÖCKER Winfried SCHMIDT Enno RENTZSCH Kristin
Year of publication	2020
Type	Article in Periodical
Magazine / Source	Journal of the American Academy of Dermatology
MU Faculty or unit	Faculty of Medicine
Citation
Web	https://www.sciencedirect.com/science/article/pii/S019096222030133X
Doi	http://dx.doi.org/10.1016/j.jaad.2020.01.049
Keywords	autoimmune bullous diseases; biochip; immunofluorescence; pemphigoid; pemphigus
Description	Background: The current standard in the serologic diagnosis of autoimmune bullous diseases (AIBD) is a multistep procedure sequentially applying different assays. In contrast, the BIOCHIP Mosaic technology combines multiple substrates for parallel analysis by indirect immunofluorescence. Methods: Sera from 749 consecutive, prospectively recruited patients with direct immunofluorescence-positive AIBD from 13 international study centers were analyzed independently and blinded by using (1) a BIOCHIP Mosaic including primate esophagus, salt-split skin, rat bladder, monkey liver, monkey liver with serosa, recombinant BP180 NC16A, and gliadin GAF3X, as well as HEK293 cells expressing recombinant desmoglein 1, desmoglein 3, type VII collagen, and BP230 C-terminus and (2) the conventional multistep approach of the Department of Dermatology, University of Lubeck. Results: In 731 of 749 sera (97.6%), specific autoantibodies could be detected with the BIOCHIP Mosaic, similar to the conventional procedure (725 cases, 96.8%). The Cohen k for both serologic approaches ranged from 0.84 to 1.00. In 6.5% of sera, differences between the 2 approaches occurred and were mainly attributed to autoantigen fragments not present on the BIOCHIP Mosaic. Limitations: Laminin 332 and laminin gamma 1 are not represented on the BIOCHIP Mosaic. Conclusions: The BIOCHIP Mosaic is a standardized time- and serum-saving approach that further facilitates the serologic diagnosis of AIBD.