Publication details

RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment

Authors

CERMAK Vladimir SKARKOVA Aneta MERTA Ladislav KOLOMAZNIKOVA Veronika PALUŠOVÁ Veronika ULDRIJAN Stjepan ROSEL Daniel BRABEK Jan

Year of publication 2021
Type Article in Periodical
Magazine / Source Biomolecules
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.mdpi.com/2218-273X/11/3/449
Doi http://dx.doi.org/10.3390/biom11030449
Keywords cancer; melanoma; metastasis; phenotype switch; amoeboid invasion
Description Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.

You are running an old browser version. We recommend updating your browser to its latest version.

More info