Publication details
Multiplexní nástroj pro rychlou detekci patogenních virů spojených s kontaminovanou vodou a prostředím
| Title in English | Multiplex tool for rapid detection of pathogenic viruses associated with contaminated water and the environment |
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| Authors | |
| Year of publication | 2021 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Viral agents have recently come to the forefront of interest, even in connection with possible contamination of water and environment. The most common consequences of the diseases associated with these contaminations are gastroenteritides manifested by fever, vomiting and watery diarrhea. However, in addition to mild febrile illness, viruses can also cause more serious conditions - respiratory infections, hepatitis, conjunctivitis, aseptic meningitis, myocarditis, encephalitis or paralysis. Currently routinely used methods for detection of these agents have their limitations. One of the most important is the limited ability to create multiplex systems to detect a large number of pathogens simultaneously. For this reason, we have developed a new detection tool that will allow to overcome this limitation. We combined multiplex ligation reaction and PCR with commercially available xMAP technology (Luminex corporation, Texas, USA). The combination of these methods allows to create open detection systems for up to 50 different targets. To detect viruses associated with food- and water-borne infections, the panel was developed and optimized, including the most discussed agents - noroviruses, hepatitis A and E viruses, adenoviruses and rotaviruses. The reliability of this panel was also verified by interlaboratory ring tests, which confirmed the limit of detection of this method, 5 × 100 to 5 × 101 genomic equivalents in the reaction. This system thus represents a fast, robust, reliable and sufficiently sensitive method for possible monitoring and control of microbiological safety of food, water and environment, and it can also serve as an aid in epidemiological investigations. In connection with the current epidemiological situation, this detection panel was expanded with additional systems targeting viruses causing respiratory diseases (the causative agents of the new disease COVID-19 and influenza A and B). |