Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

• Authors: HOFERKOVÁ Eva; KADÁKOVÁ Soňa; ŠEDA Václav; VERNER Jan; BOUDNÝ Miroslav; MLADONICKÁ PAVLASOVÁ Gabriela; LOJA Tomáš; FILIP Daniel; VEČEŘA Josef; MATULOVÁ Květoslava; SKUHROVÁ FRANCOVÁ Hana; SHARMA Sonali; ONDRIŠOVÁ Laura; PALUŠOVÁ Veronika; VOJTOVÁ Lucy; KŘEN Leoš; DOUBEK Michael; MRÁZ Marek
• Year of publication: 2023
• Type: Conference abstract
• MU Faculty or unit: Central European Institute of Technology
• Description: Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.

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