Publication details
Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the differences in the restriction patterns of all the strains exhibiting similarity 60-95%. On the other hand the ribotyping with the probe 16S-23S rDNA from E. coli led to the estimation of 4 distinct ribotypes only. The PFGE-heterogeneity of a lower degree (similarity 70-100%) was found in the strains S. haemolyticus and S. hominis, which correlates well with the estimated ribotypes. Almost each of the S. haemolyticus strains exhibited a characteristic plasmid profile corresponding to the differences observed in the antibiotics resistance patterns. Seven or 8 different plasmid profiles were found to occur in S. epidermidis and S. hominis strains, respectively. It can be concluded that the strains of all three species under study are characteristic of a considerable genomic variability. For their differentation the PFGE proved to have the highest discriminatory power. Plasmid analysis appears to be suitable as typing method for S. haemolyticus. |
| Related projects: |