Publication details
Characterisation of ribonuclease immobilised on cellulose and HEMA supports
| Title in English | Characterisation of ribonuclease immobilized on cellulose and HEMA supports |
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| Authors | |
| Year of publication | 1999 |
| Type | Article in Proceedings |
| Conference | 13th International Symposium of Affinity Technology and Bio-Recognition |
| MU Faculty or unit | |
| Citation | |
| Field | Biotechnology |
| Description | Chromatography is an integral part of large scale purification of plasmids. Samples usualy contains, except to cell components obtained after cell lysis, chromosomal DNA fragments, high molecular-weight RNA and plasmid variants. To achieve good chromatographic resolution it is necessary to degrade RNA by RNase. The aim of our work was to utilize RNase A immobilized on magnetic bead cellulose for degradation of high molecular-weight RNA before chromatographic purification of plasmid DNA. The enzyme was coupled to the particles via cyanuric chloride method. Temperature stability of soluble and immobilized RNase A was estimated after preincubation of the samples at different temperatures. Comparison of the pattern of soluble and immobilized RNase A showed a pH shift in activity. Practical use of magnetic particles in the purification of plasmid DNA is demonstrated. |
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