Publication details
Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Europen Journal of Cell Biology |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=CitationReport&qid=3&SID=W1eMKJ9l6NljDKEi3hn&page=1&doc=8 |
| Field | Other medical specializations |
| Keywords | Cryptococcus neoformans-cytoskeleton-actin-microtubules-Saccharomyces-Schizosaccharomyces |
| Description | Actin cytoskeleton and microtubules were studied in Cryptococcus neoformans. Staining with rhodamine-phalloidin revealed an F-actin cytoskeleton. Anti-tubulin TAT1 antibody revealed a microtubular cytoskeleton. Electron microscopy of chemically fixed and freeze-substituted cells revealed filamentous bundles corresponding in width to the actin microfilament cables. We conclude: (i) the topology of F-actin patches, cables and rings in C. neoformans resembles ascomycetous budding yeast Saccharomyces, while the arrangement of interphase and mitotic microtubules resembles ascomycetous fission yeast Schizosaccharomyces. The organization of the cytoskeleton of the mitotic nucleus, however, is characteristic of basidiomycetous yeasts. (ii) A specific feature of C. neoformans was the formation of a cylindrical stalk, characterized by invasion of F-actin cables and microtubules, followed by accumulation of F-actin patches around its terminal region resulting in development of an isodiametrical bud. 2009 IF=3.314. |
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