Publication details
Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Bio-Chromatography |
| MU Faculty or unit | |
| Citation | |
| Field | Biotechnology |
| Keywords | immobilized RNase A; magnetic bead cellulose; magnetic poly(HEMA-co-EDMA) microspheres; temperature stability; pH stability; plasmid purification |
| Description | A bioactive sorbent for degradation of high-molecular-weight RNA by RNase A was prepared by immobilization of RNase A on magnetic spherical carriers based on cellulose or poly(HEMA-co-EDMA). Immobilized RNase A was temperature-stable at 30-60 C. The influence of temperature on immobilized enzyme activity was also studied. A comparison of soluble and immobilized RNase A showed a pH shift in maximum of immobilized enzyme activity in dependence on the matrix type. Practical use of RNase A immobilized on magnetic bead cellulose MBC 1 for high-performance size-exclusion chromatography purification of pUC19 plasmid DNA is demonstrated. |
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