Publication details
Reversibility of cadmium-induced apoptosis is associated with increased level of telomerase activity in tobacco TBY-2 cells
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | Journal of Biomolecular Structure and Dynamics |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | cadmium-induced apoptosis; tobacco |
| Description | To monitor apoptotic changes, we followed several parameters including cell viability, DNA integrity and telomerase activity.Preapoptotic phase has been achieved by exposure of cells to 50mM cadmium for 3 days.At that time the cell culture retains more than 95% viability although the majority of DNA is fragmented into 50-200 kb.Integrity of DNA is reestablished within two days after cadmium removal suggesting that an efficient DNA repair process has occurred.In parallel, a significant increase of telomerase activity in cell extracts is observed.The data suggest an involvement of telomerase in plant chromosome healing in response to damage caused by genotoxic agents. |
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