Publication details

Chromosomes participating in translocations typical of malignant haemoblastoses are also involved in frequent interchanges induced by densely ionising radiation

Authors

LUKÁŠOVÁ Emilie KOZUBEK Stanislav KOZUBEK Michal KROHA Václav MAREČKOVÁ Andrea SKALNÍKOVÁ Magdalena BÁRTOVÁ Eva

Year of publication 2000
Type Conference abstract
MU Faculty or unit

Faculty of Informatics

Citation
Description Repeated triple-colour fluorescence in situ hybridisation was used for the detection of exchange aberrations among 10 selected chromosomes of human lymphocytes irradiated with fast neutrons and He ions of mean energy of 7 MeV and 30 MeV respectively. In each hybridisation two different pairs of chromosomes were stained. Defined stage positions of metaphases on a slide were stored on a hard disk and an automatic scan of images according to these positions was performed after six successive hybridisations. In this way, six different images of the same metaphase with differently stained pairs of chromosomes and all centromeres were obtained.
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