Publication details
Nesouhlasné výsledky pasivní hemaglutinace a imunoenzymatického stanovení specifických imunoglobulinů G v sérodiagnostice lues.
| Title in English | Conflicting results of Treponema pallidum passive hemagglutination and immunoenzymatic assay for the specific immunoglobulins G in the serodiagnostics of syphilis. |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Časopis lékařů českých |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | syphilis - serodiagnostics - passive hemagglutination - ELISA - conflicting results |
| Description | Of 2319 sera sent for the diagnostics of syphilis and examined by passive hemagglutination (TPHA) and immunoenzymatic assay for specific IgG (ELISA IgG), 141 sera (6.1 %) with conflicting results of these reactions were analyzed. Fourteen sera from infants with passively transferred maternal antibodies were put aside. Of the rest, almost four fiths of conflicting results (88 out of 111, e.g. 79.3 %) were based on the positivity of TPHA with the negativity of ELISA IgG, one fifth only (23, e.g. 20.3 %) concerned the positivity of ELISA IgG with the negativity of TPHA. The relation to syphilis was more obvious in the TPHA-positive patients: in 88 TPHA-positive patients the proven syphilitics were relatively more common (39, i.e. 44.3 %) than in 23 ELISA-positive patients (5 syphilitics, i.e. 21.7 %; p less than 0.05). Conclusion: TPHA seems to be more sensitive, whereas ELISA IgG more specific. |