Publication details
Analysis of microtubules and F-actin structures in hyphae and conidia development of the opportunistic human pathogenic black yeast Aureobasidium pullulans
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Periodical |
| Magazine / Source | Microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=W1eMKJ9l6NljDKEi3hn&page=1&doc=7 |
| Field | Other medical specializations |
| Keywords | Actin and microtubules - conidiogenesis- cytoskeleton inhibitors-budding and fission yeasts |
| Description | Cytoskeleton studied in the ascomycetous black yeast Aureobasidium pullulans, an opportunistic human pathogen, in an effort to present it as a potential target of antifungal therapy, showed long cytoplasmic microtubules. They disappeared before synchronous mitoses when intranuclear spindles originated. Actin cytokinetic rings were rare. Actin patches accumulated in apex and septum. Actin cables were not seen. During synchronous conidiogenesis, cytoplasmic microtubules extended along developing conidia, actin patches lined their subcortical areas, actin rings formed at the base of uninuclear conidia. Microtubule inhibitor methyl benzimidazol-2-ylcarbamate disintegrated microtubules, inhibited mitoses, hyphal growth and conidiogenesis. Actin inhibitor Cytochalasin D induced swelling of hyphal apexes and developing conidia, but inhibitory activity ceased after 5 to 12 h when septa appeared and conidiogenesis reappeared. The lack of unicellular organization is related to deficient actin cytoskeleton. |
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