Publication details
The Cytoskeleton during the Conidiogenesis.
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | XI. Cytoskeletální klub Vranovská Ves 23.-25.4.2003 |
| MU Faculty or unit | |
| Citation | |
| Field | Other medical specializations |
| Keywords | cytoskeleton-conidiogenesis-fungi |
| Description | Recent studies have revealed the role of cytoskeleton in the fundamental morphogenetic processes in fungi, that is, those of polar and spherical growth, and in septation during budding and fission. Actin cables are engaged in cytoplasmic transport and in cytokinesis, while actin patches take part in exocytosis and endocytosis on the cell surface. Microtubules are part of the mitotic spindle and also occur inside the cytoplasm, taking part in nuclear movement (1). Conidia - asexual spores of fungi - are relatively heterogeneous in their morphogenesis. Their cytoskeleton during the course of the conidiogenesis in fungi, has not been practically studied yet. In our communication, we will concentrate on the dynamics of cytoskeleton rearrangement in the course of blastoconidiogenesis. Our model objects were Aureobasidium pullulans (2), Cryptococcus neoformans (3) and Fellomyces fuzhouensis (4), the blastoconidiogenesis of which reveal the characteristic features. In all three organisms studied, the cytoplasmatic microtubules played a dominant role during the polar differentiation of conidia, and during nuclear movement and division. The classical role of mitotic spindles is underlined by synchronous divisions of nuclei in the multinuclear hyphae of A. pullulans, while in sterigma-forming yeast (F. fuzhouensis) the detection of mitotic spindles has been obscure. Accumulation of cortical actin patches suggests their role in the synthesis of cell surfaces. Actin cables are characteristic structures in the polar growth of conidia in the basidiomycetous yeasts. They are absent in the conidiogenesis of the representative of the ascomycetous yeast. While the actin rings have the similar role in the cytokinesis of conidia, as in the classical cytokinesis of cells, in sterigma-forming yeast the presence of actin rings has proven problematic. This is related to the specific morphogenesis in Sterigmatomycetes sp. (5), in which the processes of nuclear division and cytokinesis differ from the normal processes known today. (4). The cytoskeleton takes part in all stages of morphogenesis of the blastoconidia, as also evidenced by cessation of their development by the cytoskeleton inhibitors. References: 1. Heath, B. I. (2000). In: Actin: a Dynamic Framework for Multiple Plant Cell Functions, pp. 275-300. Edited by C. J.Staiger and others. The Netherlands: Kluwer Academic. 2. Kopecká M., Gabriel M. et al. (2003). Microbiology 149: 865-876. 3. Kopecká M., Gabriel M. et al. (2001). Eur. J. Cell Biol. 80: 303-311. 4. Gabriel M. et al. (2000). Scripta Medica 73: 341-360. 5. Fell, J. W. (1966). Ant. v. Leeuwenhoek 32: 99-104 The research was supported by grants No. 310/00/0391 and 310/03/1195 of the Grant Agency of Czech Republic. |
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