Publication details
Příprava a použití nádorového antigenu z patologického imunoglobulinu pro pacienty s mnohočetným myelomem.
| Title in English | Preparation and use of tumor antigen from pathologic immunoglobulin for patients with multiple myeloma |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Transfúze a hematologie dnes |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | monoclonal immunoglobulin; idiotypic protein; affinity chromatography |
| Description | Idiotype vaccination can be used as immunotherapy for B-cell malignancies, including multiple myeloma. Malignant plasma cells produce monoclonal immunoglobulin that can be detected in the serum and/or urine and is tumor-specific. To isolate the myeloma Id protein we used affinity chromatography with protein G column. Ig was eluted with 0,2M glycine-HCl. The protein was more than 90% pure as determined by SDS-polyacrylamide gel electrophoresis. Id protein was conjugated with KLH in presence 0,1% glutaraldehyd. The product of conjugation was applied in the preclinical experiment to take vaccinatoin as a part of preparation of clinic protocol. |
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