Publication details

Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.

Authors

ČERNOHORSKÁ Lenka VOTAVA Miroslav

Year of publication 2004
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords biofilm - antibiotics - minimum regrowth concentration - minimum inhibitory concentration
Description Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of different antibiotics was estimated in trypticase-soy broth medium after a 1-d growth on polystyrene microtiter plates. Adherent bacterial populations exhibited reduced antimicrobial susceptibility, which was not shown in submerged culture. Our results indicate that MRC can predict therapeutic outcome of antibiotic treatment better than the minimum inhibitory concentration tests commonly used.
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