Characterisation of Bifidobacterium and Lactobacillus strains in infant faeces samples

• Authors: ŠPANOVÁ Alena; RITTICH Bohuslav; KLESNILOVÁ Lenka; ŠKAPOVÁ Tereza
• Year of publication: 2005
• Type: Article in Proceedings
• Conference: BioMicroWorld-2005
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: Bifidobacterium; Lactobacillus; infant faeces; PCR; ARDRA; RAPD
• Description: In the present study genus-specific and species-specific PCR primers (targeted on a highly conserved 16S rDNA region) were used to identify Bifidobacterium and Lactobacillus strains isolated from infant faecal samples. Bifidobacteria were differentiated at the species level by a restriction fragment length polymorphism analysis using the enzymes BamHI, Sau3AI, and TaqI (amplified ribosomal DNA restriction analysis ARDRA). Bifidobacteria and lactobacilli were also differentiated using RAPD analysis. Altogether 40 strains of Bifidobacteria and 20 strains of Lactobacillus identified in the collected infant faeces were analysed.

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