Publication details
An analysis of tissues of vertebrates treated by Bromadiolone
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Toxcon 2006; 11. Interdisciplinary Slovak - Czech Toxicological Conference |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Electrochemistry; Bromadiolone; Lanirat; HPLC-ED; glassy carbon electrode; cyclic voltammetry; |
| Description | Bromadiolone is a 4-hydroxy-coumarine derivative used as rodenticide, which shows an anticoagulant effect based on the prothrombine synthesis inhibition. The determination of this compound has interest in toxicological analysis owing to its high accute oral toxicity. The dose of bromadiolone, which is expected to cause death of 50 % of male rats by oral administration (LD50) is 1.125 mg.kg-1. A determination of this compound of interest has also interest in environmental analysis taking into account its wide use to control rodent populations, which involves the resulting risk of water pollution or intoxication of non-target species. Thus we focused on suggesting of a new technique for determination of bromadiolone using high-performance liquid chromatography with electrochemical detection (HPLC-ED). Prior to analysis in flow system we have studied the basic electrochemical behaviour of bromadiolone by cyclic voltammetry on Autolab analyser (EcoChemie, Netherlands) connected with VA-Stand 663 (Metrohm, Schwitzerland). On the base of experimental results from cyclic voltammetry we designed optimal conditions for flow analysis of bromadiolone. Our analysis by high-performance liquid chromatography was carried out on CoulochemIII analyser (ESA, Inc.). Hydrogenpaladium electrode, auxiliary carbon electrode and working glassy carbon electrode were placed in electrochemical cell of volume of 50 ľl referent. We used acetate buffer (pH = 4.0):acetonitrile in ratio of 40/60 (v/v) as the most suitable mobile phase for determination of the compound of interest. We construed a hydrodynamic voltammogram, which is a dependence of peak height on applied potential. It clearly follows from the dependence that the most suitable potential for determination of bromadiolone is 836 mV. We found out that our method is sensitive enough for analysis of real samples because allows us to determine tens of nanograms of bromadiolone per ml. We utilized this optimized method to determine the amount of bromadiolone in real samples of tissues of animals which eaten bromadiolone in different forms. Earthworms (Eisenia fetida), were kept in presence of five granules of rodenticide Lanirat G in five litres of artificial soil (88 ěg/l). These earthworms were used as a feed for field mice (Microtus arvalis). Other group of field mice was fed by defined amount of bromadiolone. Earthworms kept without rodenticide and field mice fed with non-treated earthworms or fed by usual feed for rodents served as a control. We analysed the content of bromadiolone in liver of these field mice. Then we applied this method to determine the bromadiolone content in liver and stomach content of three hares (Lepus europaeus), which were found death in area where bromadiolone was applied. We found out that one them died by bromadiolone. The suggested technique is fast, sensitive and allows us to determine a trace amount of bromadiolone in biological matrix. Moreover we are able to monitor the incidence of using of bromadiolone on non target fauna. |
| Related projects: |