Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761

• Authors: ŠPANOVÁ Alena; KŘÍŽOVÁ Jana; BURDYCHOVÁ Radka; DRÁB Vladimír; RITTICH Bohuslav
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: 10th International symposium on the genetics of industrial microorganisms
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: Bifidobacterium; ARDRA; RAPD; rep-PCR; sequencing; species identification
• Description: DNA fingerprinting methods (ARDRA, RAPD, rep-PCR) have been applied for discrimination of Bifidobacterium species. Sixteen Bifidobacterium strains of eleven species which were used to evaluate the discrimination power of the above-mentioned methods were obtained from the Czech Collection of Microorganisms (CCM, Brno, Czech Republic), the American Type Culture Collection (ATCC, Manassas, USA), and the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). In characterisation of the studied strains it was found that the results obtained for the strain B. adolescentis CCM 3761 did not correspond with its taxonomic position and the strain should be reclassified as B. pseudocatenulatum. The amplicons of the 16S rDNA region of B. adolescentis CCM 3761 and B. catenulatum CCM 7094, B. pseudocatenulatum DSM 16992T, and B. adolescentis CCM 4987T were used for sequencing of DNA and for confirmation of species identification.

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