Publication details
Photochemical properties of a potential photosensitiser indocyanine green in vitro
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Photochemistry and Photobiology B: biology |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | Indocyanine green; Photosensitiser; HeLa cells; V79 Chinese hamster fibroblasts; Hydrogen peroxide |
| Description | The study investigates the photochemical properties of a potential photosensitiser, indocyanine green (ICG), in vitro. Cytotoxicity ICG was evaluated on HeLa cells and V79 Chinese hamster fibroblasts by means of MTT assay. HeLa proliferation was studied under a combined influence of effective concentration of 24 – 94 uM ICG and a laser irradiation at different energy densities. Phototoxicity was evaluated at 1, 24, and 48 hours after irradiation. No phototoxic effect was detected 1 hour after irradiation. The maximal phototoxic effect of ICG on HeLa cells was detected for an ICG concentration of 94 uM, a laser output of 360 mW, and the energy density of 99 J/cm2 24 hours after irradiation. ICG and tested by itself non-toxic 20 uM H2O2 were added at the same time to HeLa cells to enhance the phototoxic effects. 48 hours after laser irradiation a significant difference was found between the toxicity of ICG plus peroxide as compared to ICG alone. Addition of H2O2 (20 uM) causes the significant increase of phototoxicity of ICG. The results confirm that ICG could be a suitable substance for photodynamic therapy and we can potentiate its phototoxicity by addition of oxidative agent. |
| Related projects: |