Publication details

Hodnocení protinádorového efektu antigen-specifických T lymfocytů s využitím neradioaktivního testu cytotoxity

Title in English Evalution of antigen-specific lymphocytes anti-tumor effect with use of non-radioactive cytotoxicity test
Authors

ZAHRADOVÁ Lenka OČADLÍKOVÁ Darina KOVÁŘOVÁ Lucie HÁJEK Roman BULIKOVÁ Alena PENKA Miroslav MICHÁLEK Jaroslav

Year of publication 2006
Type Article in Periodical
Magazine / Source Transfuze a hematologie dnes
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords cytoxicity test; CFSE; cytotoxic T lymphocytes
Description The optimal stning concentration of CFSE has been found(1mM). Cytotoxicity has been evaluated with effector cells:target cells ratio 2:1,10:1 and 50:1 after 4, 24 and 48 hours of in vitro cultivation. Tumor specificity has been verified by 2 sets of controls: third-party PBMC as negative control target cells and expanded CFSE-labeled interferon gamma negative fraction of T cells as negative control effector cells.

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