Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; NĚMCOVÁ Petra; HORÁK Daniel
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: Ninth International Symposium on Advances in Extraction Technologies
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: DNA; isolation; magnetic microspheres; P(HEMA-co-EDMA); aqueous two-phase system; PEG 6000; NaCl
• Description: The aim of work was the isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethylmethacrylate) (P(HEMA-co-EDMA)) microspheres in the presence of an aqueous two-phase system (composed of PEG 6000 and NaCl). DNA was apparently adsorbed on magnetic particles surface from two-phase aqueous (PEG 6000/NaCl) system. The sensitivity of PCR of the DNA isolation method tested was comparable with the phenol extraction method.

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