Project information
Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering
- Project Identification
- GA203/05/2256
- Project Period
- 1/2005 - 12/2007
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Bohuslav Rittich, CSc.
- Keywords
- magnetic; microspheres; polymerization; iron oxide; stimuli-responsive.
- Cooperating Organization
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Institute of Geophysic of the SACR, v. v. i.
- Responsible person RNDr. Eduard Petrovský, CSc.
- Responsible person Ing. Daniel Horák, CSc.
Suitable magnetic liquids will be synthesized, e.g., by coprecipitation of Fe2+ and Fe3+ salts in alkaline medium, with controlled surface coverage by various stabilizing layers changing hydrophilic/hydrophobíc properties. Monodisperse stimuli-responsive polymer micro- and nanoparticles encapsulating magnetic colloid will be synthesized by (mini)emulsion and dispersion copolymerization of hydrophilic monomers with temperature and/or pH-sensitive comonomer. Multistep swelling and polymerization withprecip itation of magnetic iron oxides inside the porous rnatrix will be also used. Resulting particles will be characterized in terms of morphology, particle size and distribution, iron content, behavior in water and common solvents. Magneticcharacteristics of initial magnetic liquids and subsequently formed polymer supports will be measured in terms of optimum magnetic separation.
Publications
Total number of publications: 18
2009
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Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
Journal of Chromatography B, year: 2009, volume: 877, edition: 7
2008
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An Improved Method of Phage DNA Isolation Using Magnetic Microspheres
7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, year: 2008
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Isolation of PCR-ready DNA from lyophilisates of <I>Trichophyton</I> fungi and from phages of <I>Staphylococcus aureus</I> using magnetic microspheres
10 th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers & 10 th International Symposium on Advances in Extraction Techniques 2008, year: 2008
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Monodisperse thermosensitive poly(N-iso-propylacrylamide) microspheres for isolation of genomic DNA
10 th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers & 10 th International Symposium on Advances in Extraction Techniques 2008, year: 2008
2007
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Functionalised magnetic nano- and microspheres for molecular diagnostic applications
Biopowders; Advances in the Powder Technology, year: 2007
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Isolation of PCR-ready DNA from real samples containing PCR inhibitors
Biopowders; Advances in the Powder Technology, year: 2007
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Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system
Ninth International Symposium on Advances in Extraction Technologies, year: 2007
2006
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Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in Dairy products
6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers, year: 2006
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Hydrophilic magnetic particles for molecular diagnostics
6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers, year: 2006
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Identification of mycobacterium sp. cells using PCR after isolation of PCR-ready DNA by magnetic microspheres
26th International symposium on Separation of Proteins, Peptides and Polynucleotides, year: 2006