A simple procedure for isolation of PCR-ready bacterial DNA

• Authors: ŠPANOVÁ Alena; RITTICH Bohuslav; KŘÍŽOVÁ Jana; NĚMCOVÁ Eva; KORDAČOVÁ Lucie
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: Ninth International Symposium on Advances in Extraction Technologies
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: DNA; isolation; salting-out; NaCl
• Description: PCR-ready DNA was isolated using salting-out procedure from crud cell lysates. The evaluated method is suitable for DNA isolation from pure cultures of bacterial cells. The reproducibility of RAPD and rep-PCR paterns was comparable with phenol extraction procedure.

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