Functionalised magnetic nano- and microspheres for molecular diagnostic applications

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; HORÁK Daniel; BENEŠ Milan
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: Biopowders; Advances in the Powder Technology
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzymes immobilization; PCR-ready DNA; amplification methods
• Description: Magnetic microspheres (P(HEMA-co-EDMA), P(HEMA-co-GMA), and PGMA with hydrophilic properties were prepared by single-step dispersion polymerisation. Sterically or electrostatically stabilised colloidal magnetite was obtained by precipitation of Fe2+ and Fe3+ salts and encapsulated by dispersion (co)polymerisation of HEMA and GMA. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNA, magnetic separation of Salmonella cells or degradation of their intracellular inhibitors was demonstrated. Genomic DNA was successfully isolated from cell lysates on weakly acid derivatives of magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres in the presence of PEG 6000 and sodium chloride.

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