Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins

• Authors: RYVOLOVÁ Markéta; PEŠ Ondřej; FOHLEROVÁ Radka; HEJÁTKO Jan; PREISLER Jan
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: CECE 2007 4th international interdisciplinary meeting on bioanalysis
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: capillary electrophoresis; GFP
• Description: In this work, using laser diode (405 nm) as a prospective excitation source for monitoring of phosphorylation process of cytosolic histidine-containing phosphotransfer proteins (designated as AHP1-6 in Arabidopsis) has been suggested. To our knowledge, this laser emitting at 405 nm has not been applied for CE-LIF of GFP and/or GFP-tagged proteins yet.

Related projects

• Molecular basis of cell and tissue regulations
• Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine