Publication details
In silico and In vitro Protein Engineering as a Bioanalytical Tool
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | CECE 2007 |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | protein enginnering lectin in silico |
| Description | Protein engineering is a procedure of developing useful or valuable proteins by mutations of residues. It is usually performed in vitro, when the original DNA is mutated, incorporated into host cells (usually E. coli) and the resulting protein with mutated residues is expressed. It can also be performed in silico, when mutations are performed on a model in a computer. Our effort is to connect both approaches to increase efficiency of the procedure. In our case, the engineered molecules are called lectins, nonenzymatic proteins that recognize carbohydrates. The lecture will be mainly focused on in silico part of the procedure, which is performed by the program TRITON developed in our laboratory. During this procedure, mutants are automatically generated, refined and their affinity and specificity is predicted. The method is then complemented by in vitro approaches that finish with thermodynamic and kinetic measurements. |
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