Publication details
Testy únikového chování s půdními bezobratlými
| Title in English | Avoidance tests with soil invertebrates |
|---|---|
| Authors | |
| Year of publication | 2008 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Soil invertebrates occur world-wide. They participate on humus decomposition, transport of mineral particles and nutriet cycle in soil. The representants of main groups (earthworms, enchytraeids, nematodes, springtails, isopods, etc.) are sufficiently sensitive to environmental pollutants and stress conditions of soil compartment (changes in soil humidity, salinity, pH, lack of food). From these reasons, they are used as model organisms in some ecotoxicological tests interested in adult surviving and reproduction. However, these tests last several weeks. Faster, two-days, avoidance test could be so their suitable supplement. This screening method is based on pressumption that organisms dispose the receptors sensitive to stress. They are so able to avoid or escape a contaminated substrate. The number of individuals in tested substrate versus control soil (natural soil, artificial soil) is analysed in its. Avoidance test could be in addition used in ecotoxicological research (potential contamination, effects of individual polutants) as well as in ecological studies (preference/occurence/absence organisms at various localities). The avoidance test with two enchytraeid species (E.albidus, E. crypticus) was optimalised at the workplace of authors and avoidance test was verified in various types of studies interested in physical-chemical soil properties (artificial soil x natural soils) and contamination (pesticide carbendazim). The results indicated higher avoid reaction for E.albidus than E.crypticus and higher preference of natural soils in opposition to artificial soil. The escape reaction of enchytraeids increased with increasing concentration of carbendazim in soil (EC50 = 7.63 mg/kg). The avoidance test was also used in an experiment with solid wastes (ash, contaminated wood and soil). The irritant effect of waste mixtures was increased from soil to ash. |
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