Off-Line Coupling of Capillary Electrophoresis to Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Elemental Speciation

• Authors: PREISLER Jan; PEŠ Ondřej; FOLTYNOVÁ Pavla; VYHNÁNEK Radek; VACULOVIČ Tomáš; KANICKÝ Viktor
• Year of publication: 2008
• Type: R&D Presentation
• MU Faculty or unit: Faculty of Science
• Description: On-line coupling of column separation techniques with inductively coupled plasma optical emission/mass spectrometry (ICP OES/MS) is routinely used for sensitive, element-selective analysis. Direct linking to ICP usually relies on a nebulizer, which often suffers from reduced separation resolution due to induced liquid flow. Here we report first off-line coupling of capillary electrophoresis (CE) with ICP MS, which is based on eluent deposition on a suitable target followed by laser ablation (LA) and transfer into ICP. The technique is compatible with microcolumn separations, such as capillary electrophoresis or nano liquid chromatography, in terms of both sensitivity and resolution. Furthermore, the fractions collected on the target can be archived and analyzed using other techniques, e.g. MALDI MS or laser-induced fluorescence.

Related projects

• Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
• Molecular basis of cell and tissue regulations
• Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface
• Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine