Application of Aqueous Phase System and Hydrophilic Magnetic Microspheres for Isolation of Bacterial PCR-ready DNA from Dairy Products

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; ŠÁLEK Petr; TRACHTOVÁ Štěpánka; HORÁK Daniel
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: 7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: DNA; isolation; PCR; aqueous phase system; magnetic microparticles; dairy products
• Description: The isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres in the presence of an aqueous phase system (composed of PEG 600 or PEG 6000 and NaCl)was studied. The results presented show that magnetic microparticles are suitable for the isolation of genomic DNA which can by used for PCR. Proposed method was applied to analysis of real dairy products.

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