Publication details
Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | The International Journal of Biological Markers |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | alphaB-crystallin; biomarker; ProteinChip; renal cell carcionoma; SELDI-TOF MS |
| Description | Spectrometric-based surface-enhanced laser desorption/ionization ProteinChip (SELDI-TOF) facilitates rapid and easy analysis of protein mixtures and is often exploited to define potential diagnostic markers from sera. However, SELDI-TOF is a relatively insensitive technique and unable to detect circulating proteins at low levels even if they are differentially expressed in cancer patients. Therefore, we applied this technology to study tissues from renal cell carcinomas (RCC) in comparison to healthy controls. We found that different biomarkers are identified from tissues than those previously identified in serum, and that serum markers are often not produced by the tumors themselves at detectable levels, reflecting the nonspecific nature of many circulating biomarkers. We detected and characterized alphaB-crystallin as an overexpressed protein in RCC tissues and showed differential expression by immunohistochemistry. |
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