Molekulární typizace vybraných kmenů bakterií rodu Lactobacillus ze sbírky Laktoflora

• Title in English: Molecular typing of selected bacterial strains of genus Lactobacillus from Culture Collection of dairy microorganisms Laktoflora
• Authors: DUŠKOVÁ Marta; ŠPANOVÁ Alena; DRÁB Vladimír; RITTICH Bohuslav
• Year of publication: 2008
• Type: Article in Periodical
• Magazine / Source: Mlékařské listy–Zpravodaj
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: Lactobacillus; typing; rep-PCR; RAPD
• Description: Rep-PCR with (GTG)5 primer and RAPD with M13 primer were used for typing of selected strains of genus Lactobacillus collected in Czech Collection of Dairy Microorganisms (CCDM) Laktoflora. Rep-PCR with (GTG)5 primer was more suitable than RAPD with M13 primer. Typing enabled to identified 1 strain into species L. acidophilus and 15 strains in L. helveticus. These results were confirmed with species specific PCRs. They were in accord with biochemical identification in 88% of strains.

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